Table 1.

Comparison of astrocyte differentiation from hPSCs

Differentiation study	Duration (days)	Efficiency (GFAP positive)	Astrocyte markers	Method (Neuralization)	Additions in Medium (expansion phase)	Maturation (astrocyte differentiation)	Function test
Krencik et al., 2011	180	>90 %	GFAP S100β CD44 NF1A	EB	EGF FGF2 (Suspension)	CNTF, LIF or 10% FBS	Astrocyte-neuron co-culture Glutamate uptake Propagation of calcium
Emdad et al., 2012	35	50-80%	GFAP A2B5 S100β	EB	N2 FGF EGF CNTF	N2 CNTF	Transwell migration
Gupta et al., 2012	70	>90%	GFAP S100β EAAT1 EAAT2	EB	EGF FGF2 Heparin (Suspension)	BMP2,BMP4, LIF	Glutamate uptake
Juopperi et al., 2012	120	-	GFAP S100β	EB	Astrocyte medium contain FBS (Monolayer)	FBS	-
Lafaille et al., 2012	90	-	GFAP	Dual-smad inhibition	EGF FGF2	5% FBS	-
Roybon et al., 2013	>80	>90%	CD44 S100β, CX43 Vimentin NF1A aldolase C	Dual-smad Inhibition	B27 NTFs FBS (Monolayer)	FGF1 or FBS	Glutamate uptake
Serio et al., 2013	>70	>90%	Vimentin, NF1A GFAP S100β EAAT1	EB with dual- smad inhibition	EGF LIF->EGF FGF2 (Suspension)	CNTF	Glutamate uptake Astrocyte-neuron co-culture Calcium Wave
Shaltouki et al., 2013	>70	60-80%	CD44 GFAP NF1A S100β EAAT1	EB	Actinvin A Heregulin 1 β IGFI FGF2 (Monolayer)	CNTF BMP2	Glutamate uptake Astrocyte-neuron co-culture