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. Author manuscript; available in PMC: 2016 Nov 29.
Published in final edited form as: Methods Cell Biol. 2016 May 2;135:121–147. doi: 10.1016/bs.mcb.2016.04.008
I-SceI Digestion:
0.5 µL 10× I-SceI buffer (NEB)
1 µL 500 ng/µL donor plasmid DNA
2 µL 5 U/µL I-SceI (NEB)
1.5 µL Nuclease-free water
Incubate 37°C, 1 h
Store on ice, 4°C