sgRNA with Cas9 protein: | |
2 µL | 1 µg/µL sgRNA |
1.2 µL | 5 µg/µL Cas9 protein (PNA BIO) |
1 µL | 0.5% phenol red |
0.8 µL | Nuclease-free water |
TALEN mRNA solution: | |
1 µL | 500 ng/µL left TALEN mRNA |
1 µL | 500 ng/µL right TALEN mRNA |
1 µL | 0.5% phenol red |
2 µL | Nuclease-free water |