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. Author manuscript; available in PMC: 2016 Nov 29.
Published in final edited form as: Methods Cell Biol. 2016 May 2;135:121–147. doi: 10.1016/bs.mcb.2016.04.008
sgRNA with Cas9 protein:
2 µL 1 µg/µL sgRNA
1.2 µL 5 µg/µL Cas9 protein (PNA BIO)
1 µL 0.5% phenol red
0.8 µL Nuclease-free water
TALEN mRNA solution:
1 µL 500 ng/µL left TALEN mRNA
1 µL 500 ng/µL right TALEN mRNA
1 µL 0.5% phenol red
2 µL Nuclease-free water