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. 2016 Nov 11;113(47):E7629–E7638. doi: 10.1073/pnas.1612872113

Fig. S7.

Fig. S7.

Immunofluorescent images of the control and FtsZ2-1–overexpressing cells showing the localization of cyclin B. In both strains, CYCLIN B-3HA was integrated into the CYCLIN B locus. Cells were synchronously cultured and were heat shocked at hour 8 for 2 h, as shown in Fig. 4. Cyclin B was detected with the anti-HA antibody. (Left) The change in the level and localization of cyclin B in the control cells during cell-cycle progression. The cell-cycle stage was defined based on the cell shape according to ref. 25. (Right) The localization of cyclin B in the control and FtsZ2-1–overexpressing (FtsZ OX) cells at hour 16 in the synchronous culture. Magenta, autofluorescence of the chloroplast; green, immunostained cyclin B-3HA; cyan, DNA stained with DAPI; PC, phase-contrast image. (Scale bars: 1 µm.) Two independent experiments produced similar results. The results from one experiment are shown.