Primers used for plasmid construction |
Z_Bgl_Bam_Inf_F |
CAGATCTGGAGGATCC ATGAACCACCGTACGCCTC |
Z_Not_Inf_R |
TGCAGCCGGGCGGCCGCTTACTTTTGGAATCGACG |
184_HSpro200_Inf_F |
GTCCCGAGGGTGACG CTTATAGCTTACGTGGCGG |
Nos_Inf_R |
TCATGTTTGACAGCTTATCATCG |
CMD184C_+25∼_R |
CGTCACCCTCGGGACTTGATGTTTACGTTC |
Nos_CmUra_Inf_F |
AGCTGTCAAACATGA GAACTGAGGGGCGAACGC |
D184(1270)F |
ACACGAATCACACGGTGCTG |
D184(+1448)R |
TTGCCGATAACGCAGAAGAGA |
CycA_−1620_F |
CCGGTTATGGCCTATGGAGC |
CycA_+2018_R |
TTATCGCCTTCTGGACCACTG |
CycA_1482_R_HA |
AACATCGTATGGGTA GCGCCCGAAAGGGTTTTCGCTC |
CycA_+1_F_Ura |
AGTCAGCTGCTAGGG TGAACGATAACTTGAACGTGTGTGGGATTC |
HA_4_F |
TACCCATACGATGTTCCTGACTATG |
Ura_+471_R |
CCCTAGCAGCTGACTGTATC |
CycA_−1506∼_F |
GACGTGCGGGTAGGGAACG |
CycA_∼+1991_R |
CTTCTGGACCACTGGCAGCG |
HSPro_F |
CTTATAGCTTACGTGGCGGATTCGCAGCG |
Z_∼1512_R |
TTACTTTTGGAATCGACGCAGAAAATCC |
Z_CycA3′_Inf_F |
CGATTCCAAAAGTAA TGAACGATAACTTGAACG |
HSpro_Ura_Inf_R |
CACGTAAGCTATAAG CCCTAGCAGCTGACTGTA |
CycB_−536∼_F |
GATCCCATCCATCGGGCCAGTTC |
CycB_∼+1994_R |
TCAGTAACCAGAAGCTCCTCGA |
Ura_CycB3′_Inf_F |
AGTCAGCTGCTAGGG GTCGAGACCGCTGCGCTCCG |
HA_F |
TACCCATACGATGTTCCTGACTATGCG |
CmUra3′+471_Inf_R |
CCCTAGCAGCTGACTGTATC |
Z_CycB3′_Inf_F |
CGATTCCAAAAGTAA GTCGAGACCGCTGCGCTCCG |
HA_CycBORF_Inf_R |
AACATCGTATGGGTA GAACAGGTTCGCGACACCGA |
CDKB_−951∼_F |
CGGGATCGCGTGGAAGGAGC |
CDKB_∼+1966_R |
TCCTGCATCTGGAAGCGAGCTC |
Ura_CDKB3′_Inf_F |
AGTCAGCTGCTAGGG CGGTCGTCACTGCATCCGAT |
HA_CDKBORF_Inf_R |
AACATCGTATGGGTA CGGCAGCATGACCTCGTTCT |
Z_CDKB3′_Inf_F |
CGATTCCAAAAGTAA CGGTCGTCACTGCATCCGAT |
BamHI-mORANGE-F |
TGAACTCAGAGGATCCATGGTGAGCAAGGGCGAG |
BamHI-mORANGE-R |
CGTATGGGTAGGATCCCTTGTACAGCTCGTCCATG |
CatP_Inf_F |
AGCAGATAGTTCATAGAGTTAGGAGGGC |
D184_Nos_R |
TCGCTGAGCGGTTTC TCATGTTTGACAGCTTAT |
CatP_Ura_inf_R |
TATGAACTATCTGCT CCCTAGCAGCTGACTGTA |
D184C_+28∼_F |
GAAACCGCTCAGCGACCA |
pET100_FtsZ2-1_F |
CACCAACCACCGTACGCCTCTTTCG |
pET100_FtsZ2-1_R |
TTACTTTTGGAATCGACGCAGAAAATC |
Primers used for quantitative RT-PCR |
PCNA_CMS101C_qRT_F |
CTCCGTGCGCATTTCTGTAA |
PCNA_CMS101C_qRT_R |
TCGTCACCACGAACCGTAAA |
CycA_CMI203C_qRT_F |
TCACGCGAGGTTCTGCAA |
CycA_CMI203C_qRT_R |
CTTTGGCTGCAACGAGCAT |
Cdc20_CMA138C_qRT_F |
CAGTAGGGACCGCCTTTGG |
Cdc20_CMA138C_qRT_R |
CGCTTGTTCGCTTCAACATC |
CycB_CMI227C_qRT_F |
TGCAACTCGTCGGCGTTA |
CycB_CMI227C_qRT_R |
TCGGGCGCGTATATTTCC |
CDKB_CMH128C_qRT_F |
ACGCATGAGGTTGTGACTTTGT |
CDKB_CMH128C_qRT_R |
ACGCGTCCCGAGAAGGA |
DRP3(1981)F |
GCAGGCGAAGTATCGAGC |
DRP3(2050)R |
AGTAACTCGCAAGAAGCGTCT |