Fig. S4.

Interactions of activated Dictyostelium Ras family GTPases with commonly used pan-Ras probes. (Left) Pan-Ras probes Raf1-RBD and Byr2-RBD interact with a whole variety of active Ras family members in the Y2H assay. (Right) Specificity of relevant interaction was confirmed by the use of dominant-negative variants. Yeast were transformed with the indicated constructs and selected for the presence of prey and bait plasmids by growth on DD media lacking leucine and tryptophan. Interactions were assayed by growth on stringent TD media additionally lacking histidine in the presence of 3 mM 3-AT or highly stringent QD media lacking leucine, tryptophan, histidine, and adenine.