Fig. S6.

RasB⁻ cells display no major defects in macropinocytosis and phagocytosis of bacteria. (A) Growth of rasB⁻ cells in liquid medium was moderately impaired compared with WT cells. A representative growth curve of three independent experiments is shown. (B) Impaired phagocytosis of bacteria by rasB⁻ cells as assessed by clearance of E. coli strain B/r in the supernatant. A representative curve of three independent experiments is shown. (C) Accumulation of TRITC-dextran by macropinocytosis after 90 min of incubation was visualized by confocal microscopy. (Scale bar: 10 μm.) (D) Quantification of TRITC-dextran uptake by macropinocytosis revealed no major defects in the rasB⁻ mutant (mean ± SD, n = 2).