Table S1.
Oligonucleotides used in this work
| Primer | Sequence | Orientation |
| Dictyostelium constructs | ||
| GFP-ForG-FL | ||
| ForG-BU | 5′-CGCGGGATCCGCATGATATTATCAATTACATTTCAATTAGATC-3′ | Forward |
| ForG-2600-BglD | 5′-ATCAAAGATCTCCATGGAAAAGTATCCAAAAACTATG-3′ | Reverse |
| ForG-800-NsiUp | 5′-AAGTGAATGCATTGATTTTGGTTTCCAAATC-3′ | Forward |
| ForG-SD-FL | 5′-CGCGTCGACTTATTTATTTAAATTTAATTGTGATCC-3′ | Reverse |
| GFP-ForG∆DAD | ||
| ForG-PstI-DWN | 5′-AGGACCTGCAGCAATTCAACC-3′ | Forward |
| ForG-DelDAD-SD | 5′-CGCGTCGACTTATGGATCAGCACCACCAGCAATCTTTTTA-3′ | Reverse |
| pDM304-YFP | ||
| DYFP-BamHI-F1 | 5′-ACCGGATCCAAAAATGAGTAAAGGTGAAGAACTTTTC-3′ | Forward |
| DYFP-BglII-R1 | 5′-AATAGATCTGAGTCCGGATTTGTATAGTTCATCCATG-3′ | Reverse |
| YFP–ForG-N | ||
| ForG(1–423)-BglII-F1 | 5′-ATTAGATCTATGATATTATCAATTACATTTC-3′ | Forward |
| ForG(1–423)-SpeI-R1 | 5′-AATACTAGTTTAAATTTTCTTTTCAAATTCATTAA-3′ | Reverse |
| YFP-ForG-N∆RBD | ||
| ForG-delRBD-BU | 5′-ACTGGATCCTCTTCAAAATGGGTAAAAGC-3′ | Forward |
| ForG(423)-SpeD | 5′-ACAACTAGTTTAAATTTTCTTTTCAAATTCATTAA-3′ | Reverse |
| YFP-ForG∆RBD∆DAD | ||
| ForG-delRBD-BU | 5′-ACTGGATCCTCTTCAAAATGGGTAAAAGC-3′ | Forward |
| ForG(1–1,040)-SpeI-R2 | 5′-CGCACTAGTTTATGGATCAGCACCACCAGC-3′ | Reverse |
| LifeAct-mRFP | ||
| Lifeact_N-BglII-F1 | 5′-GATCTAAAAATGGGTGTCGCTGACCTGATAAAGAAGTTTGAAAGC | Forward |
| ATCTCCAAGGAAGAGA-3′ | ||
| Lifeact_N-SpeI-R1 | 5′-CTAGTCTCTTCCTTGGAGATGCTTTCAAACTTCTTTATCAGGTCAGC | Reverse |
| GACACCCATTTTTA-3′ | ||
| ForG-KO | ||
| ForG BU KO | 5′-CGCGGGATCCGCATGATATTATCAATTACATTTCAATTA-3′ | Forward |
| ForG PstD KO | 5′-CGCCTGCAGCATTATATCTTTTTCATTTG-3′ | Reverse |
| ForG HU KO | 5′-GCCAAGCTTCTTCCTCTTCTTCAAATACTTC-3′ | Forward |
| ForG Sal KO | 5′-CGCGTCGACTTGGAATAACCAAGCTACTAAACGTT-3′ | Reverse |
| Bsr-AU | 5′-CAGTTACTCGTCCTATATACG-3′ | Forward |
| KO-AD4 | 5′-GTTCTTGAGCGACATTCATAG-3′ | Reverse |
| RasB-KO | ||
| RasB-5KO-BU | 5′-CGCGGATCCCAATTCCAATAGTAAAAAGTC-3′ | Forward |
| RasB-5KO-PD | 5′-GCGCTGCAGGATAGTAAGTGCACTCTTACTG-3′ | Reverse |
| RasB-3KO-H3U | 5′-GCGAAGCTTGTCAAGATGATTACAGTGCTA-3′ | Forward |
| RasB-3KO-SD | 5′-CGCGTCGACCTTTGATCTGCCTGGCTCTTTG-3′ | Reverse |
| RasB-KO-AD | 5′-CTAAAGGATTAAACAATCACCACCT-3′ | Reverse |
| YFP-RasB | ||
| RasB-BglU | 5′-GCGAGATCTATGTCAGTTTCAAATGAATATAAAT-3′ | Forward |
| RasB-SpeD | 5′-GCGACTAGTCTAAAGGATTAAACAATCACCACC-3′ | Reverse |
| YFP-RasG | ||
| RasG-BglU | 5′-GCGAGATCTATGACAGAATACAAATTAG-3′ | Forward |
| RasG-SpeD | 5′-GCGACTAGTTTATAAAAGAGTACAAGCTTTTAATGG-3′ | Reverse |
| VN210-RasB-G15V/-S20N | ||
| RasB-BglU | 5′-GCGAGATCTATGTCAGTTTCAAATGAATATAAA-3′ | Forward |
| RasB-SpeD | 5′-GCGACTAGTCTAAAGGATTAAACAATCACCACC-3′ | Reverse |
| YFP-Raf1-RBD | ||
| mRaf(RBD)_BglU | 5′-GTAAGATCTGATTCTTCTAAGACAAGCAATACT-3′ | Forward |
| mRaf1(RBD)_SpeD | 5′-GATACTAGTTTAATCCAAAAAATCCACTTGCAG-3′ | Reverse |
| E. coli constructs | ||
| ForG-3P (562–1,074) | ||
| ForG BamHI 3P | 5′-CGCGGATCCCCAATTTCTGGTGGTGGTGCA-3′ | Forward |
| ForG SD | 5′-GCGGTCGACTTATTTATTTAAATTTAATTGTGATCCTCCAGATGAATTTTCAGG-3′ | Reverse |
| ForG-1P (599–1,074) | ||
| ForG BamHI 1P opt | 5′-GCGCGGATCCGGAGCACCGCCACCGCCCCCTCCGCCACCGCC | Forward |
| TCCGGGTGGTAAAAAAGCAGGAGCACCA-3′ | ||
| ForG SD | 5′-GCGGTCGACTTATTTATTTAAATTTAATTGTGATCCTCCAGATGAATTTTCAGG-3′ | Reverse |
| ForE-4P (1,009–1,561) | ||
| ForE-C_BU | 5′-GGATCCATTTCTGGTGCTCCACCCCCACCCCCA-3′ | Forward |
| ForE CProFull_SD | 5′-GGCGTCGACTTAATTTTTATTTGGACTTGT-3′ | Reverse |
| S. cerevisiae constructs | ||
| AD–ForG-N (1–423) | ||
| ForG pGADT7 EcoRI | 5′-GCCGAATTCATGATATTATCAATTACATTTC-3′ | Forward |
| ForG pGADT7 BamHI | 5′-GGCGGATCCTTAAATTTTCTTTTCAAATTCATTAA-3′ | Reverse |
| AD-Raf1-RBD (50–132) | ||
| mRaf1-GAD-RU | 5′-CGCGAATTCGGA GATTCTTCTAAGACAAGCAATACT-3′ | Forward |
| mRaf1-GAD-BD | 5′-CGCGGATCCTTAATCCAAAAAATCCACTTGCAG-3′ | Reverse |
AD, Gal4-activation domain; BD, Gal4-binding domain.