Table S2.

GTPase variants used for Y2H in this study

GTPase	Inactive	Amino acids	Dictybase gene ID
RasB G15V ∆CAAX	Yes	1–193	DDB_G0292998
RasB S20N ∆CAAX
RasC G13V ∆CAAX		1–185	DDB_G0281385
RasD G12V ∆CAAX	Yes	1–183	DDB_G0292996
RasD S17N ∆CAAX
RasG G12V ∆CAAX	Yes	1–185	DDB_G0293434
RasG S17N ∆CAAX
RasS G12V ∆CAAX		1–190	DDB_G0283537
RasU G25V ∆CAAX		1–209	DDB_G0270138
RasW G20V ∆CAAX		1–212	DDB_G0270122
RasX G20V ∆CAAX		1–209	DDB_G0270124
RasY G21V ∆CAAX		1–212	DDB_G0270126
RasZ G20V ∆CAAX		1–210	DDB_G0270140
RapA G14V ∆CAAX		1–182	DDB_G0291237
RapB G34V ∆CAAX		1–201	DDB_G0272857
RheB Q64L ∆CAAX		1–181	DDB_G0277041
Rac1A G12V ∆CAAX		1–190	DDB_G0277869
Rac1B G12V ∆CAAX		1–190	DDB_G0268622
Rac1C G12V ∆CAAX		1–189	DDB_G0282365
RacA G12V ∆C		1–319	DDB_G0286555
RacB G12V ∆CAAX		1–189	DDB_G0279605
RacC G15V ∆CAAX		1–188	DDB_G0293526
RacD G17V		1–254	DDB_G0291976
RacE G20V ∆CAAX		1–219	DDB_G0280975
RacF1 G12V ∆CAAX		1–189	DDB_G0269176
RacF2 G12V ∆CAAX		1–189	DDB_G0276967
RacG G12V ∆CAAX		1–197	DDB_G0269178
RacH M13V ∆CAAX		1–196	DDB_G0269240
RacI S14V ∆CAAX		1–201	DDB_G0277897
RacJ D18V ∆CAAX		1–205	DDB_G0292560
RacL G12V ∆CAAX		1–192	DDB_G0292816
RacM Y14V ∆CAAX		1–185	DDB_G0289103
RacN G62L ∆CAAX		1–215	DDB_G0278009
RacO G13V ∆C		1–213	DDB_G0277791
RacP G58V		1–376	DDB_G0285453
RacQ A56L ∆CAAX		1–181	DDB_G0278011