Skip to main content
. 2016 Nov 9;113(47):13378–13383. doi: 10.1073/pnas.1616627113

Fig. 2.

Fig. 2.

Selectivity for packaging of Cap1G RNAs from cells with altered RNA proportions. (A) Schematic representation of constructs with altered numbers of guanosines at the U3/R junction used to skew intracellular RNA populations. ΔU3, R + pA indicates replacement of downstream LTR sequences with an SV40 polyadenylation signal. (B) Schematic representation of the portion of the HIV-1 genome to which riboprobe 2 is complementary. Note the five guanosines at the U3/R border, which allowed discrimination among more products than the 3G riboprobe. (C) RNase protection assay of constructs with altered numbers of guanosines. Lane 1: undigested probe. Lane 2: size standards. Lanes 3–8: fragments protected by RNA standards. Lanes 9–16: products protected by the indicated cell and virus RNA samples from cells transfected with the indicated HIV-1 GPP derivatives. Mobilities of products protected by Cap1G, Cap2G, Cap3G, and Cap4G RNAs are indicated at the right.