Fig. 3.

Analysis of HIV-1 RNA forms associated with polyribosomes. RNase protection assay on cell lysate fractions from chronically infected CEM-SS cells, analyzed with riboprobe 1 (Fig. 1). Lane 1: undigested probe. Lane 2: size standards. Lanes 3–6: fragments protected by the indicated RNA standards. Lanes 7–8: fragments protected by chronically infected cell medium (virus) and total cell lysate. Lanes 9–12: probe fragments protected by RNA from 10, 20, 30, and 40% sucrose step gradient fractions. Mobilities of protected products from the 5′ and 3′ ends of viral RNA are indicated on the right.