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(A) Spin-down assay showing that Cut7 N-terminal domain C-terminally fused to GFP (circles) binds to MTs, whereas GFP alone (open squares) does not. The buffer comprised 25 mM PIPES pH 6.8, 30 mM NaCl, 7 mM MgCl2, 1 mM EGTA, and 1 mM 2-mercaptoethanol. (B) MT sliding driven by kinesin-1 dimers fused to the Cut7 N terminus. MTs move slowly (Upper) in KPEM100 gliding buffer, and faster (Lower) in KPEM100 + 250 mM NaCl gliding buffer, 1 mM ATP.
