Fig. 4.
Determination of RNC stability by pulse proteolysis. (A) DHFR V75R RNCs. Blue indicates protein on the ribosome; gray, protein off the ribosome. (B) RNase H I53D RNCs. Green indicates on the ribosome; gray, off the ribosome. (C) Barnase W35F/W94F/H102M RNCs. Purple indicates on the ribosome; gray, off the ribosome. Each gel and trace shown is representative of three separate experiments. *Incomplete tRNA digestion. #RNase A. Error bars represent the SD of the Cm for each curve determined by three separate experiments, except for Barnase, for which the Cm is the average of two experiments.