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Methotrexate (MTX) binds in vitro translated DHFR V75R. (A) Gray indicates the fraction folded as a function of urea for in vitro translated DHFR V75R. Blue indicates the fraction folded as a function of urea for in vitro translated DHFR V75R in the presence of 2 μM MTX. (B) Gel of MTX used for quantitation in A.
