Fig. S2.

The presence of the ribosome does not inhibit protease accessibility. (A) TEV cleavage of DHFR V75R-TEV-(GS)5-SecM as a function of time after RNase A digestion overnight. (B) Quantification of gel band intensities normalized to the highest band intensity on the gel. The original band is shown as filled circles; the band corresponding to cleavage product, as open circles. (C and D) Same as A and B, respectively, but as RNCs. Each gel and point shown is representative of three separate experiments. Error bars represent the SD of the normalized band intensity for each time point.