Fig. S3.

Purification of labeled RNCs. IVT reactions (input) were loaded onto a sucrose cushion and centrifuged as described in Materials and Methods. Supernatant (sup) was aspirated, and the pellets were washed three times (W1, W2, and W3) with 200 μL of ice-cold HKM+DTT. Pellets were then resuspended, mixed with SDS/PAGE loading dye, and loaded on a gel.