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Comparison of IVT and recombinant protein
| Protein | Cm, purified (urea, M) | ∆Gunf, purified (kcal mol−1) | Cm, IVT (urea, M) | ∆Gunf, IVT (kcal mol−1) |
| DHFR V75R | 2.50 ± 0.18 | 4.50 ± 0.32 | 2.10 ± 0.16 | 3.78 ± 0.29 |
| RNase H I53D | 2.21 ± 0.09 | 4.41 ± 0.18 | 2.16 ± 0.10 | 4.31 ± 0.19 |
