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Determination of RNC stability
| Protein | Cm, on (urea, M) | Cm, off (urea, M) | ∆Gunf, on (kcal mol−1) | ∆Gunf, off (kcal mol−1) | ∆∆Gunf, on-off (kcal mol−1) |
| DHFR V75R-(GS)5-SecM | 1.81 ± 0.05 | 2.58 ± 0.11 | 4.57 ± 0.14 | 6.50 ± 0.25 | −1.93 ± 0.29 |
| RNase H I53D-(GS)5-SecM | 1.65 ± 0.10 | 2.14 ± 0.13 | 4.07 ± 0.23 | 5.29 ± 0.33 | −1.22 ± 0.16 |
| Barnase W35F/W94F/H102M-(GS)5-SecM | 2.09 ± 0.01 | 2.31 ± 0.04 | 3.90 ± 0.02 | 4.32 ± 0.08 | −0.42 ± 0.08 |
