Fig. S3.
Current traces for BEST1TripleA. (A and B) Current traces, using the same bilayer, from which I–V relationships presented in Fig. 1C are derived. The dashed line represents the zero current level. (A) Asymmetric (30 mM cis, 10 mM trans) and (B) symmetric (30 mM cis, 30 mM trans) KCl conditions. (C and D) Current traces, from the same bilayer, in the presence of ∼300 nM free Ca2+ (C) and after the addition of 10 mM EGTA to the cis and trans chambers (D). The protocols used are identical to those described for BESTWT in Fig. 1 A–C.