Fig. S5.
The lyotropic sequence of anion permeability is unaltered in the BEST1TripleA mutant. (A) Example I–V relationships recorded using voltage steps from −100 to +100 mV. Following the procedure outlined in Fig. 1G, after first recording using symmetric 30 mM KCl (black I–V trace), the solution on the trans side was replaced (by perfusion) with solutions containing 30 mM KBr, KI, or KSCN. I–V traces are shown for data collected using the standard voltage protocol. (B) Permeability ratios were determined by solving the Goldman–Hodgkin–Katz equation (SI Extended Data Analysis) using three separate experiments to compute the SEM.