Fig. S6.

The F80I mutation does not make BEST1 a sodium-selective ion channel. (A and B) Experiments are performed as in Fig. 2A except bath solutions contained NaCl instead of KCl. Example I–V relationships are shown for WT (A) and F80I (B). (C) Using reversal potentials measured from both the 30/10 and 30/90 mM KCl conditions, the Goldman–Hodgkin–Katz voltage equation was solved (SI Extended Data Analysis) to compute an average permeability ratio per experiment. Error bars are computed using the SEM from three separate experiments.