Fig. S7.
BEST1TripleA has a higher unitary Cl− transport rate than BEST1WT. To compare the activity of BEST1WT and BEST1TripleA, channel protein (purified and incorporated into liposomes in parallel) was incorporated into liposomes using a 1:1,000 protein:lipid ratio and the fluorescence-based Cl− flux assay was carried out as described in Fig. 2E (Methods). The arrow indicates when the CCCP proton ionophore was added to initiate the assay. In comparison to the other flux assays, 10-fold less protein was used in this assay in order to better compare the relative activities of BEST1WT and BEST1TripleA.