Absence of vasculature at p0 in both Itgb1f/f; Pitx1-cre and Itgb1f/f; Prop1-cre pituitary glands. (A and B) Immunostaining of coronal sections with independent markers, CD31 and PLVAP, failed to detect endothelial cells in Itgb1f/f; Pitx1-cre and Itgb1f/f; Prop1-cre pituitary glands at birth. A, anterior lobe; I, intermediate lobe; P, posterior lobe; PLVAP, plasmalemmal vesicle-associated protein. (Scale bars: 130 μm.)