Fig. 3.

Endothelial cells invade Itgb1^f/f; Pitx1-cre pituitary glands at e13.5 but fail to recruit pericytes. Serial sagittal sections beginning at the midline and progressing through the lateral anterior lobes immunostained with VEGFR2 (endothelial cells) and PDGFRβ (pericytes) are shown. Pericytes recruited to endothelial cells in Itgb1^f/f control pituitary glands (filled arrowheads) are shown. Endothelial cells that failed to recruit pericytes in Itgb1^f/f; Pitx1-cre pituitary glands (empty arrowheads) are shown. Magnified images of the same panels are provided in Fig. S5D. (Scale bar: 62.5 μm.)