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. 2016 Nov 3;113(47):13408–13413. doi: 10.1073/pnas.1614970113

Fig. S3.

Fig. S3.

Timing of endocrine cell differentiation is normal, but spatial alterations and changes in gene expression levels occur in Itgb1f/f; Pitx1-cre and Itgb1f/f; Prop1-cre embryos. (A) Ventral expression of POMC in Itgb1f/f; Pitx1-cre pituitaries at e13.5. (B) Thyroid-stimulating hormone-β (TSH-β) in Itgb1f/f; Pitx1-cre pituitaries at e15.5. (C) Altered spatial expression of POMC and GH in Itgb1f/f; Pitx1-cre pituitaries at p0. (D) qPCR of pituitary mRNA from Itgb1f/f; Pitx1-cre pituitaries at p0 revealed reduced GH and Prl but increased TSH-β. (E) Reduced density of GH cells and mislocalized dorsal TSH-β cells in Itgb1f/f; Prop1-cre pituitaries at p18 immunostained for Pit-1 and PH3, GH and Prl, TSH-β, and LH-β. (F) qPCR of pituitary mRNA from Itgb1f/f; Prop1-cre at p17 mimics GH and Prl data from Itgb1f/f; Pitx1-cre pituitaries at p0. Midsagittal sections are shown in A-C, and coronal sections are shown in E. (Scale bars: A–C and E, 130 μm.) KO, knockout; WT, wild type.