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. 2016 Nov 3;113(47):13408–13413. doi: 10.1073/pnas.1614970113

Fig. S7.

Fig. S7.

Deletion of epithelial integrin β1 alters ECM. (A) RNA-seq reads mapped to the Itgb1 locus on the UCSC Genome Browser indicate absence of exon 3-encoded sequence in RNA from e12.5 Itgb1f/f; Pitx1-cre dissected pituitaries. (B) FN deposition and reduced fibril formation (arrows) in e12.5 Itgb1f/f; Pitx1-cre pituitary glands. Asterisks indicate rostral aspect of the anterior lobe where endothelial cells first invade. (Scale bar: 32.5 μm.) (C) VEGFR2 and PDGFRβ immunostaining at e14.5 in VEGF-Af/f control and VEGF-Af/f; Pitx1-cre knockout littermate pituitaries showed comparable endothelial cell invasion and recruitment of pericytes (arrows). (D) RNA in situ hybridization images from the genepaint.org website showing e14.5 pituitary gland expression of VEGF-A and VEGF-C ligands and VEGF receptors (VEGFR-1, VEGFR-2, and VEGFR-3). Arrows indicate that both VEGF-A and VEGF-C bind to the highly expressed VEGFR2.