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Mutating residues in the cytoplasmic gate increases the sensitivity to permeant and impermeant sulfhydryl reagents. Cells were assayed for [3H]TBZ binding ability following incubation with the indicated concentrations of (A) MTSEA, (B) MTSET, and (C) MTSES. Results presented are averages of at least two repeats, each point in duplicate; error bars indicate SE.
