Fig. S4.

Mutating the gating residues changes the sensitivity to MTSEA. Cells were assayed for [³H]TBZ binding ability following incubation with increasing concentrations of MTSEA. (A) Mutations at position 423: wild-type (solid black square), Y423A (solid blue circle), Y423S (solid red triangle), and Y423F (green star). (B) Mutations at position 419: wild-type (solid black square), Y419A (solid blue circle), Y419S (solid red triangle), and Y419F (green star). (C) Mutations at position 222: wild-type (solid black square), M222S (solid red triangle), M222L (solid purple circle), and M222F (green star). (D) Mutations at position 218: wild-type (solid black square), R218A (solid blue circle ), and R218H (solid orange triangle ). Results presented are averages of at least two repeats, each point in duplicate; error bars indicate SE.