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Pictures of the encapsulation process. (A) PEG substrates (black arrow) are placed in sterile glass wells. (B) The PVA/cell solution is poured in the wells. (C) Glass wells after incubation at 37 °C for 3 h. (D) Glass wells are soaked in a large excess of growth medium. (E) The obtained PVA hydrogel films (white arrow) are collected and rinsed in PBS solutions. (F) The films (white arrow) are stored individually in cell culture wells and incubated in growth medium at 37 °C.
