Fig. 5.

SCFA supplementation increases IGF-1 and decreases bone mass in antibiotic-treated mice. (A) Experimental procedure schematic. Mice were treated with broad-spectrum antibiotics for 2 wk to perturb microbiota, followed by supplementation of antibiotic water with either SCFA (Abx+SCFA) or sodium chloride control (Abx) for another 4 wk. Control (Ctrl) group received water with NaCl for 6 wk. (B) Serum IGF-1 and IGFBP3 levels. (C) Trabecular bone mass was determined by micro-CT. (D and E) Tissue IGF-1 production. (D) Abdominal fat pad and (E) liver total tissue IGF-1 (Left) was calculated by multiplying the IGF-1 production per gram tissue (Middle) by the weight of the tissue (Right). *P < 0.05; **P < 0.01; ns, not statistically significant.