Fig. S9.

Effects of antibiotics and SCFA on IGF-1 levels are consistent across antibiotic mixtures. SPF mice were treated as outlined in Fig. 5A, but antibiotic treatment was with antibiotic mixture 2, described in the Materials and Methods. (A) Serum IGF-1 and IGFBP3 levels. (B) Trabecular bone mass determined by micro-CT. (C and D) Tissue IGF-1 production (Left) was calculated by multiplying IGF-1 production per gram tissue (Middle) by the weight of the tissue (Right) for both liver (C) and fat pad (D). *P < 0.05; **P < 0.01; ns, not statistically significant.