Fig. 2.

Cdc45 forms a complex with Gins15 and Gins23 in vivo. (A) Partial purification of the CG complex from extracts by successive affinity, size exclusion, and anion exchange chromatography; a silver-stained gel of the peak fractions from the final column is shown. (B) Western blotting was performed on the peak fractions from A, using the indicated antisera. (C) Yeast 2 hybrid analyses of interactions between the N- and C-terminal domains of Gins15 and full-length Gins23 and Cdc45. Growth on the selective plates (lacking histidine) is indicative of interaction.