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ChIP reactions using synchronized SacCdc45 cells were used to examine genomic localization of Cdc45 and MCM during the cell cycle. (A) Flow cytometry profiles of asynchronous, G1/early S-phase, S-phase, and G2-enriched synchronized cell populations are indicated. (B–E) ChIP profiles showing the enrichment of DNA in the immunoprecipitates at the various genomic loci at the indicated times.
