Table S1.
Oligonucleotides used in this study
| Primer name | Primer sequence 5′–3′ | Primer description |
| TG75 | TTTCTGCAGGTTAGTGAGATAATATCCTATCC | Saci_0177 upstream cloning F primer |
| TG76 | TTATTAATTTTTGTTCTGCTGCACTGAAATCTTTATTTTCAACTTTTATC | Saci_0177 upstream cloning R primer |
| TG77 | AGCAGAACAAAAATTAATAAGTGAAGAAGATTTACATCATCATCATCATCATTAAGATTTCCACTGAAGCTAG | Saci_0177 downstream cloning F primer |
| TG78 | TTTGGTACCTCTTCAGCGACATCACAAGC | Saci_0177 downstream cloning R primer |
| TG134 | AAAGTTATCCACGGACGCCG | qPCR F primer 1,727-kb region (ter) |
| TG135 | TGGCGACCAACAAATGGTTAGAT | qPCR R primer 1,727-kb region (ter) |
| TG136 | CCACGGTACTCCAAATCTTTATCA | qPCR F primer 429-kb region |
| TG137 | GTTGGAAGTTATCTGCGTTACCG | qPCR R primer 429-kb region |
| TG164 | CCCGATAACAGAGACTTTACTCG | qPCR F primer 579-kb region (oriC1) |
| TG165 | GATGAGATTGACGCACTTGTGAAA | qPCR R primer 579-kb region (oriC1) |
| TG172 | AGAAAGTCACCCAGGATATAGCG | qPCR F primer 0/2,226-kb region (oriC2) |
| TG173 | GAAGGAGTCCATTATTTGCACAAG | qPCR R primer 0/2,226-kb region (oriC2) |
| TG174 | TTGATAAGTGCCATCTTCCCG | qPCR F primer 1,197-kb region (oriC3) |
| TG175 | AGCCACAAATCTCAACCTGGATA | qPCR R primer 1,197-kb region (oriC3) |
| TG176 | CGTCTTGCCATATTTTAGTGCG | qPCR F primer 104-kb region |
| TG177 | AATTGCGTACTCTCCTTTAGGTCA | qPCR R primer 104-kb region |
| TG180 | TCCTGGAGGAGAAATAGGGAT | qPCR F primer 250-kb region |
| TG181 | TGTATACGCGCATGAATCAC | qPCR R primer 250-kb region |
| TG182 | TTGAATGGGCAATTTGGTAA | qPCR F primer 700-kb region |
| TG183 | CGTTAACGGTATACGTCACGA | qPCR R primer 700-kb region |
| TG186 | CACCAGTGGCACTTTACTCG | qPCR F primer 850-kb region |
| TG187 | TCAGGGCTACGCATTAGTGA | qPCR R primer 850-kb region |
| TG188 | AATGTACAAATTTCACCGACAGA | qPCR F primer 1,000-kb region |
| TG189 | CCTTAACTCCATTTGCCACA | qPCR R primer 1,000-kb region |
| TG190 | GACTATGCAGCTGGTCCTGA | qPCR F primer 1,350-kb region |
| TG191 | ATTTACGGAATTGGCGTAGG | qPCR R primer 1,350-kb region |
| TG192 | TCCATTTGTGGATTTCTTTGA | qPCR F primer 1,500-kb region |
| TG193 | GCACTTGGGAACATAAGGTG | qPCR R primer 1,500-kb region |
| TG194 | GGTGTTTCATGACGGAGAGA | qPCR F primer 1,900-kb region |
| TG195 | AGCCAAAGGAGCACCTAAAC | qPCR R primer 1,900-kb region |
| TG196 | GCAGACATAAGGAACGGGAT | qPCR F primer 2,050-kb region |
| TG197 | CCATCCTAATCACGCATTTG | qPCR R primer 2,050-kb region |
| Cdc45-F-NdeI | CGCCGCATATGGAACTATTCTTAGGAGAGCCCA | Amplification of SisCdc45 |
| Cdc45-R-SalI | CTAGTCGACTGTTCCGATAAGATTCTTAATTCTATC | Amplification of SisCdc45 |
| MCM-N-F-EcoRI | CCGAATTCCATGGAAATTCCTAGCAAACAGATTGAC | Construction of MCM-GST fusions |
| MCM-N-R-XhoI | TTCAATGCTCGAGTCATGAAACTTCTATGCTACTAACTTTCATA | Construction of MCM-GST fusions |
| MCM-N-A domain-R-XhoI | GCTAACCTCGAGTCAAGGTATTCCTACAATTCTAACGTGAA | Construction of MCM-GST fusions |
| MCM-N-BC domain-F-EcoRI | CCGGAATTCTAGAGTTATAGAACTTAGAAAAATAAGAAGTAC | Construction of MCM-GST fusions |
| SH TOP | CTCTATCTATACTCTATCTATATAAGTATTTAAACTTTACTCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | Substrate for EMSA and helicase assays |
| SH LOWER | TTTTTTTAGAGTAAAGTTTAAATACTTATATAGATAGAGTATAGATAGAG | Substrate for EMSA and helicase assays |
| ssDNA | TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGAGTAAAGTTTAAATACTGGTATAGCTAGAGTCTAGATAGAG | ssDNA added to ATPase assays |
| ssDNA TOP | CTCTATCTAGACTCTAGCTATACCAGTATTTAAACTTTACTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | For use in pulldown assay |
| ssDNA LOWER | TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGAGTAAAGTTTAAATACTGGTATAGCTAGAGTCTAGATAGAG | For use in pulldown assay |
| MCM 5′ | GAATTCCCATGGAAATTCCTAGCAAACAGATTGACTATAG | For mcm gene amplification and cloning into pET33b |
| MCM delta A 5′ | GAATTCCCATGGAAATTCCTAGCAAACAGATTGACTATAG | For amplifying DNA corresponding to MCM(106-686), MCM-ΔA, and cloning into pET33b |
| MCM 3′ | GATCGTCGACTTAGACTTTTTTGTAACATTCTGGTTTTGCTTCGT | For mcm gene amplification and cloning into pET33b |