Sensitivity to 5FOA |
Detects ability of rpb1 LEU2 plasmid to complement rpb1Δ [37] |
Resistance to drug. RPB1 LEU2 plasmid complements rpb1Δ |
Sensitivity to drug (Partial or no complementation of rpb1Δ by rpb1 LEU2)
|
Suppressor of Ty (Spt-) |
lys2-128∂; reports on chromatin defects and start site selection [52]. Specific class of GOF Pol II mutants [37,41,53]. |
Lysine auxotroph (Lys-) |
Lysine prototroph (Lys+) |
Mycophenolic acid sensitivity (MPAS) |
IMD2 expression required for resistance; reports on start site selection [57,58]. Specific classes of GOF and LOF Pol II mutants [37,41,56]. |
Resistance to drug |
Sensitivity to drug for GOF mutants, relative resistance for LOF mutants. |
Modulation of transcriptional readthrough at gal10Δ56 (GalR)
|
gal10Δ56; likely reports on termination, mRNA processing and initiation [37,41,54,55]. It is found widely in LOF Pol II mutants, some GOF. |
Moderate sensitivity to galactose (GalS) |
Resistance to galactose (GalR) |