Fig. 5.

Effect of NPS 2143 on pre-contracted tone and VGCC activity. (A) Mean data showing that NPS 2143 induced vasorelaxations of methoxamine-induced pre-contracted endothelium removed vessels. Note that NPS 2143-induced vasorelaxations of KCl-induced contractions are attenuated compared to the effects on methoxamine-induced contractions. n=4 animals used per experiment, n=3 vessel segments per animal. **P<0.01, ***P<0.001, ****P<0.0001, 2-way ANOVA followed by Bonferroni post hoc test. (B) Representative trace and (C) mean data showing that increasing concentrations of NPS 2143 inhibited whole-cell VGCC currents. n=7 patches from n=4 animals. **P<0.01, ****P<0.0001, Student's t-test.