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. 2016 Nov 29;11(11):e0167336. doi: 10.1371/journal.pone.0167336

Fig 2. Characterization of mGEnC-1-derived GAGs reveals RNA as a major contaminant.

Fig 2

The RNA content in extracts from conditionally immortalized mouse glomerular endothelial cells (mGEnC-1) was visualized by ethidium bromide agarose gel electrophoresis (A), and barium acetate gel electrophoresis (B) before and after RNase treatment. Enzymatic degradation of RNA in mGEnC-1 GAG extracts removes the RNA band observed on ethidium bromide gel, and a large spot that appears to co-migrate with HS on the barium acetate gel.