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. 2016 Nov 29;11(11):e0167336. doi: 10.1371/journal.pone.0167336

Fig 3. Glycosaminoglycans (GAG) extracts contain significant RNA impurities, which can be digested by RNase-I treatment.

Fig 3

Visualizing nucleotide impurities in GAG preparations from conditionally immortalized mouse glomerular endothelial cells (mGEnC-1), human umbilical vein endothelial cells (HUVEC), immortalized retinal pigmental epithelial cells (ARPE-19) (A), and mouse kidney, heart, liver and spleen (B) on ethidium bromide agarose gels, revealed significant RNA contaminations. RNase-I treatment efficiently removed the contamination from mGEnC-1 and ARPE-19 GAGs, though minor impurities remained in HUVEC-, kidney- and spleen-derived extracts.