Fig 1. Schematic presentation of the study design.

A) RNA of brain tissue from six naïve animals was extracted and pooled to create an identical dilution series. cDNA synthesis was then performed with each of the four kits. B) RNA of brain tissue from 6 naïve animals and 6 animals suffering traumatic brain injury was extracted. Each sample was reverse-transcribed, using all four kits, again ensuring the exact same starting material for each kit.