Fig 7. Distinct roles of various receptors in SAA-induced secretion of cytokines/chemokines.

Thioglycollate-elicited primary macrophages were isolated from wild-type C56BL/6 or mutant mice respectively deficient in TLR2, TLR4, TLR2/RAGE, or TLR4/RAGE. Following stimulation with SAA (0.5 μg/ml) for 16 h, the extracellular levels of cytokines and chemokines were determined by Cytokine Antibody Arrays. Because the SAA-induced cytokine profiles were superimposable between TLR4- and TLR4/RAGE-deficient macrophages, we only provided results for TLR4 KO macrophages. The experiment was repeated twice. *, P < 0.05 versus “-SAA” controls; #, P < 0.05 versus “WT + SAA”.