Figure 5. CD46 locus is frequently coamplified with 1q21, and copy gain for either MCL1 or CD46 identifies patients with high CD46 expression.

(A) About 30% of newly diagnosed patients demonstrate 1q21 copy gain overlapping the MCL1 locus by array comparative genomic hybridization (CGH), defined as log₂ copy number >0.3, and a similar proportion demonstrate amplification along the 1q arm, including CD46 (gray shaded histogram; left axis). High frequency of coamplification along the 1q arm with MCL1 amplification is shown by the purple line and quantified on the right axis (n = 322 patients). (B) Dot plot of log₂ CGH values in patient samples indicates that copy numbers for CD46 and MCL1 loci are highly correlated and cluster in regular copy number intervals (n = 322 patients). (C) Mean CD46 transcript expression values (quantified as fragments per kilobase per million mapped reads [FPKM]) for CD46 or MCL1 copy-amplified cohorts, defined by ≥0.3 log₂ CGH value, versus nonamplified cohorts indicate high CD46 expression in MCL1 or CD46 amplified patient samples, compared with total population or samples without copy gain (n = 260 patients). Data represent mean ± 95% CI. One-way ANOVA with Tukey’s multiple comparison correction, **P < 0.01, ***P < 0.001, ****P < 0.0001.