Table 1. Guide to modifying the DIA method.
| Goal | Selectivity |
m/z Range Covered |
Number of Sample Injections |
Chromatographic Sampling Rate |
|---|---|---|---|---|
| Increase sensitivity | ||||
| Reduce isolation width | ↑ | ↓ | ||
| Reduce isolation width and use multiple injections per sample |
↑ | ↑ | ||
| Increase max ion inject time* | ↓ | |||
| Increase resolving power and max ion inject time* |
↑ | ↓↓ | ||
| Sample more peptides | ||||
| Use multiple injections per sample |
↑ | ↑ | ||
| Increase isolation width | ↓ | ↑ | ||
|
Improve chromatogram sampling |
||||
| Increase isolation width | ↓ | ↑ | ||
| Reduce m/z range covered | ↓ | ↑ |
*
Instruments with an ion trap mass analyzer that use automatic gain control (e.g. Thermo Scientific LTQ, Velos, Orbitrap, and Exactive series).