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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Sep 14;1865(1):121–131. doi: 10.1016/j.bbapap.2016.09.004

Figure 5. Effect of Bcl-2Δ21 on the distribution of SERCA3b in sucrose-density gradient fractions.

Figure 5

Microsomes isolated from SERCA3b-overexpressing HEK-293 cells were incubated with Bcl-2Δ21 (molar ratio of SERCA3b:Bcl-2Δ21 ~1:28) (A) and without Bcl-2Δ21 (B) at 37°C for 1 hr followed by sucrose density gradient separation. Fractions (1–9) from the sucrose gradient were collected and analyzed by Western blotting with the PL/IM430 antibody. Higher molecular weight bands that are above SERCA3b bands show aggregation of SERCA3b.