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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Sep 14;1865(1):121–131. doi: 10.1016/j.bbapap.2016.09.004

Figure 9. Co-immunoprecipitation of SERCA3b with Bcl-2.

Figure 9

Co-immunoprecipitation was carried out using microsomes of HEK-293 cells overexpressing SERCA3b (Vec) and microsomes of HEK-293 cells overexpressing SERCA3b and Bcl-2 (Bcl-2) with the anti-Bcl-2 antibody followed by Western blotting with PL/IM430 antibody. Data from two different microsome isolations (first and second) are shown. Lane 1 and 2 show the first experiment and Lane 3, 4, 5 and 6 show the second experiment. Lane 5 and 6 show control samples run without the anti-Bcl-2 antibody.