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. Author manuscript; available in PMC: 2017 Nov 29.
Published in final edited form as: Circulation. 2016 Sep 29;134(22):1738–1748. doi: 10.1161/CIRCULATIONAHA.115.020086

Figure 4.

Figure 4

A. Total RyR2 abundance measured by Western blot (control n=6, sarcomere-mutation HCM n=16, sarcomere-negative HCM n=7). B. Phosphorylated RyR2 abundance by Western blot for the CaMKII/protein kinase A phosphorylation site (pS2808-RyR2) and the CaMKII-specific phosphorylation site (pS2814-RyR2). C. Total ryanodine receptor binding following addition of [3H]ryanodine to cardiac tissue homogenates. Control n=6, sarcomere-mutation HCM n=11 (MYBPC3-mutation n=7, MYH7-mutation n=4), sarcomere-negative HCM n=9. D. Relative change in RyR2 binding specific counts with pre-incubation with either H2O2 (oxidation) or spermine NONOate (nitrosyl donor).