Table 3.
Measurements of arousal-indicative behaviors and the correlated bioelectrical characteristics of NGC neurons that changed in Trends over P3-P6 of age.
| Items observed | P3 | P4 | P5 | P6 | Trenda | P3 vs P6b | ||
|---|---|---|---|---|---|---|---|---|
| Arousal- related behaviors (all n=14) |
Movement duration (sec) |
109.6 ± 18.6 |
119.2 ±14.9 |
226.0 ± 28.7 |
336.6 ± 31.8 |
p< 0.05 | p<0.001 | |
| Movement distance (cm) |
1.27 ± 0.18 |
1.62 ± 0.44 |
4.11 ± 0.60 |
5.42 ± 0.62 |
p< 0.05 | p<0.001 | ||
| Latency to movement (sec) |
119.3 ± 26.4 |
42.1 ± 7.4 |
21.4 ± 2.7 |
20.3 ± 3.7 |
p< 0.05 | p<0.005 | ||
| Quiescence duration (sec) |
7 9 0 .4 ± 18.6 |
780.8 ± 14.9 |
674.0 28.7 |
563.4 ± 31.8 |
p< 0.05 | p<0.001 | ||
| Bioelectrical characteristics of NGC neurons |
Action potential |
Neurons with AP train (% ) |
17 (2/12) |
33 (6/18) |
38 (6/16) |
80 (12/15) |
p< 0.05 | p<0.01c |
| Amplitude (mV) |
47.7 ± 2.0 (8) |
49.0 ± 3.0 (6) |
52.0 ± 2.9 (8) |
56.0 ± 2.5 (14) |
p< 0.05 | p<0.05 | ||
| Threshold (mV) |
−30.8 ± 1.8 (8) |
−34.2 ± 1.0 (6) |
−37.2 ± 2.1 (8) |
−38.2 ± 2.4 (13) |
p< 0.05 | p<0.01 | ||
| Currents by single voltage step |
Inward (pA) |
−1366 ± 144 (12) |
−2099 ± 292 (18) |
−2207 ± 291 (16) |
−2689 ± 270 (15) |
p< 0.05 | p<0.001 | |
| Outward (pA) |
1427 ± 353 (12) |
3220 ± 598 (18) |
4132 ± 730 (16) |
5312 ± 852 (15) |
p< 0.05 | p<0.001 | ||
| Currents by multiple voltage steps |
Neg Pk (pA) Vh −70 mVe |
−1110 ± 243 (12) |
−2075 ± 27 (18) |
−2389 ± 351 (16) |
−3161 ± 443 (15) |
p< 0.05 | p<0.001 | |
| Neg Pk (pA) Vh −40 mV |
−1273 ± 88 (12) |
−1667 ± 250 (18) |
−1790 ± 209 (16) |
−2461 ± 344 (15) |
p< 0.05 | p<0.01 | ||
| Steady State Current (pA) |
3160 ± 299 (12) |
4447 ± 389 (18) |
4473 ± 515 (16) |
4829 ± 438 (15) |
p< 0.05 | p<0.01 | ||
| RSrd, Any item vs any others | all p<0.05. | |||||||
a
Mann-Kendall test for Trend, one-tailed.
b
ANOVA, two-tailed.
c
χ2 test, 2-tailed.
d
Spearman rank correlation coefficient, one-tailed. Each and any of the behavior measures is significantly correlated with each and any of the neuronal characteristics.