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UVB irradiation induces production of proinflammatory chemokines. Ten week old female MRL/lpr mice were irradiated as described above. RNA was isolated from irradiated mice (n=5) and unmanipulated controls (n=5), and analyzed for various chemokines by PCR (top panel). Chemokine levels in skin lysates from irradiated MRL/lpr mice (n=5) and unmanipulated MRL/lpr controls (n=4) were measured using a flow cytometer based multiplex assay (bottom panel). *P<0.05, **P<0.01, ****P<0.0001.
