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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Virology. 2016 Oct 27;500:103–113. doi: 10.1016/j.virol.2016.10.013

Figure 8.

Figure 8

Phylogenetic tree shows the evolutionary relationships between 47 viral concatenated amino acid sequences (7762 gap-free sites). The Maximum Likelihood (ML) tree was constructed using the MEGA 6.0 software with the ML algorithm and default setting. The bar length represents 0.2 substitutions per amino acid site. The proposed new OSy chlorovirus species is indicated in red and resides between the two separate phylogenetic subclades of NC64A viruses – one subclade contains PBCV-1 while the other subclade contains chlorovirus NY-2A. Both subclades share almost perfect gene colinearity and they replicate in the same host. Branch support was estimated from 1000 bootstrap replicates. Four Ostreococcus virus sequences serve as outgroups to root the tree.