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. 2016 Nov 30;7:1930. doi: 10.3389/fmicb.2016.01930

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Copyright © 2016 Huerta-Uribe, Marjenberg, Yamaguchi, Fitzgerald, Connolly, Carpena, Uvell, Douce, Elofsson, Byron, Marquez, Gally and Roe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of AHU1 on stx2::GFP expression, growth rate and lysis of E. coli. ZAP0273 transformed with pNY16 was cultured in the presence of 25-200 μM AHU1, and induced with 1 μg/ml MMC. Expression of stx2::GFP expression was measured by fluorescence, and bacterial growth by OD₆₀₀. (A) Inhibition of MMC-induced stx2::GFP expression by AHU1. (B) Effect of AHU1 on bacterial growth in the absence of MMC. (C) Inhibition of MMC-induced bacterial lysis by AHU1. Experiments were performed in triplicate, and data plotted as the mean with standard deviation from the mean displayed by error bars.