Table 1.
Structural and subcellular localization analysis of the EgTIPs.
| Ar/R selectivity filter∗ | NPA motif∗∗ | Froger’s positions | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Protein | Cha | Size | pI | MW (kDa) | TMb | Locc | H2 | LC | H5 | LE1 | LE2 | LB | LE | P1 | P2 | P3 | P4 | P5 |
| EgTIP1.1 | 3 | 260 | 5.56 | 26 | 6 | Vacuole | H | F | I | A | V | NPA | NPA | T | S | A | Y | W |
| EgTIP1.2 | 10 | 252 | 4.68 | 26 | 6 | Vacuole | H | F | I | A | V | NPA | NPA | T | S | A | Y | W |
| EgTIP1.3 | 2 | 252 | 4.79 | 26 | 6 | Vacuole | H | F | I | A | V | NPA | NPA | T | S | A | Y | W |
| EgTIP1.4 | 10 | 251 | 5.16 | 25.8 | 6 | Vacuole | H | F | I | A | V | NPA | NPA | T | S | A | Y | W |
| EgTIP2.1 | 6 | 248 | 5.52 | 25 | 6 | Vacuole | H | H | I | G | R | NPA | NPA | T | S | A | Y | W |
| EgTIP2.2 | 4 | 250 | 5.12 | 25 | 6 | Vacuole | H | H | I | G | R | NPA | NPA | T | S | A | Y | W |
| EgTIP2.3 | 4 | 259 | 6.78 | 26.4 | 6 | Cell membrane/vacuole | H | G | F | G | R | NPA | NPA | A | S | A | H | |
| EgTIP3.1 | 11 | 262 | 6.7 | 27.5 | 6 | Vacuole | H | F | I | A | L | NPA | NPA | T | A | A | Y | W |
| EgTIP3.2 | 8 | 259 | 6.04 | 27.8 | 6 | Vacuole | S | L | I | A | R | NPA | NPA | T | A | A | Y | W |
| EgTIP4.1 | 3 | 243 | 5.91 | 25.3 | 6 | Vacuole | H | H | I | A | R | NPA | NPA | T | S | A | Y | W |
| EgTIP5.1 | 11 | 256 | 6.81 | 25.7 | 6 | Cell membrane/vacuole | N | F | V | G | C | NPA | NPA | T | S | A | Y | W |
∗Ar/R: aromatic/arginine; H2: transmembrane helix 2; LC: loop C; H5: transmembrane helix 5; LE: loop E; ∗∗NPA: Asn-Pro-Ala. aChromosome location of the corresponding gene. bNumber of transmembrane (TM) helices predicted by TMpred. cBest possible subcellular localization predicted by Plant-mPloc.